Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and
research findings. The notebook for the 4 modules are displayed separately.
Potassium Sensor
June
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
July
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
August
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040