Team:HKUST-Rice/Notebook

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Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for the 4 modules are displayed separately. Potassium Sensor June June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 July June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 August June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040

Phosphate Sensor June June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 July June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 August June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040

Nitrogen Sensor June June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 July June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 August June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040

Expression Platform June June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 July June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 August June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040