Team:HKUST-Rice/Notebook

• 
• 
• HOME
• PROJECT
  • OVERVIEW
  • POTASSIUM
    • P_kdpF
    • MODELING
  • PHOSPHATE
  • NITRATE
    • P_dcuS
    • P_yeaR
  • PARALLEL SENSORS
    • P_araBAD characterization
  • APPLICATION
  • DIY GEL IMAGING
• ACHIEVEMENT
  • PARTS
  • MEDAL REQUIREMENTS
• TEAM
  • MEMBERS
  • ADVISORS
  • INSTRUCTORS
  • ATTRIBUTIONS
• HUMAN PRACTICES
  • INTRODUCTION
  • EXPLORATORY RESEARCH
  • BIOETHICAL DEBATE
• WETLAB
  • NOTEBOOK
  • PROTOCOL
  • SAFETY
• 
• 

Notebook

Potassium Sensor June June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 July June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 August June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040

Phosphate Sensor June June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 July June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 August June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040

Nitrogen Sensor June June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 July June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 August June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040

Expression Platform June June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 July June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040 August June 9 Wet lab · Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600 · Transformation of BBa_B0034 and BBa_E0040 into DH10B June 10 Wet lab · Found reference promoter J23101 in BBa_I20260 from part registry · Looked for Pcombox gene sequence from the genomic DNA · Bacterial inoculation of BBa_B0034 and BBa_E0040