Team:HKUST-Rice

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• HOME
• PROJECT
  • OVERVIEW
  • POTASSIUM
    • P_kdpF
    • MODELING
  • PHOSPHATE
  • NITRATE
    • P_dcuS
    • P_yeaR
  • PARALLEL SENSORS
    • P_araBAD characterization
  • APPLICATION
  • DIY GEL IMAGING
• ACHIEVEMENT
  • PARTS
  • MEDAL REQUIREMENTS
• TEAM
  • MEMBERS
  • ADVISORS
  • INSTRUCTORS
  • ATTRIBUTIONS
• HUMAN PRACTICES
  • INTRODUCTION
  • EXPLORATORY RESEARCH
  • BIOETHICAL DEBATE
• WETLAB
  • NOTEBOOK
  • PROTOCOL
  • SAFETY
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ABSTRACT

Nitrogen (N), Phosphorus (P), and potassium (K) are three macronutrients for plants, and deficiencies in any of these can lead to plant diseases. By creating a biological sensor that can quickly provide soil status to plant owners, we can prevent plant diseases due to the lack of nutrients.

In the previous iGEM competitions, multiple teams have worked on nitrate and phosphate responsive promoters, but none of them attempted to provide a potassium responsive promoter. In view of this, our team is constructing a biological sensor in E. coli, which can detect NPK levels in the surrounding environment and give responses in the form of colors. In addition, we are characterizing the effects of a dual output system, in contrast to a single output system, in order to anticipate the expression of multiple outputs in a single system.

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Home	Project			Result	Achievement	Team	Human Practice	Wetlab	Future Work
	Overview Nitrate Description Design Experiments Modelling Results Discussion	Phosphate Description Design Experiments Modelling Results Discussion	Potassium Description Design Experiments Modelling Results Discussion	Nitrate Phosphate Potassium Expression	Parts Medal Requirement	Collaboration Attribution Team Members Advisors Instructors	Debate Exploratory Research	Notebook Protocol Safety	Application Improvement