Difference between revisions of "Team:TJU/Description"

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<h2> Project Description </h2>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
 
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<li> A clear and concise description of your project.</li>
 
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
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<li>References and sources to document your research.</li>
 
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<h4>Advice on writing your Project Description</h4>
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    <div id="header-2"><img src="https://static.igem.org/mediawiki/2015/6/66/Project_9.11.png" width="1100px" alt=""/></div>
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    <a href="https://2015.igem.org/Team:TJU/Overview"><img src="https://static.igem.org/mediawiki/2015/7/79/Overview-p1.png" width="119"  alt=""/></a> <a href="https://2015.igem.org/Team:TJU/Background"><img src="https://static.igem.org/mediawiki/2015/9/94/Background-p1.png" width="119"  alt=""/></a></br></br>
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    <a href="https://2015.igem.org/Team:TJU/Design"><img src="https://static.igem.org/mediawiki/2015/1/14/Design-p1.png" width="119" alt=""/></a> <a href="https://2015.igem.org/Team:TJU/Results"><img src="https://static.igem.org/mediawiki/2015/4/43/Result-p1.png" width="119" alt=""/></a></div>
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    </br>
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    <div align="center"><h2> Overview </h2></div>
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    <p>Microbial fuel cells(MFCs) are capable of converting the chemical energy stored in the chemical compounds to electrical energy with the aid of microorganisms. Compared with traditional fossil fuels, MFCs have sorts of advantages, such as no emissions of polluting gas, mild reactive conditions and flexible applications in extreme conditions. </p></br>
  
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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</br><a href="https://static.igem.org/mediawiki/2015/e/ee/Background_11.png" target="_blank" ><img src= "https://static.igem.org/mediawiki/2015/e/ee/Background_11.png"  width="680"  alt=""/></a>
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<div id="Enlarge">         
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<p> <b>Figure 1.</b> <span style="font-size: 14px"> The overall relationship of three kinds of bacteria in our co-culture system.</span><a href="https://static.igem.org/mediawiki/2015/e/ee/Background_11.png"  target="_blank"><img src="https://static.igem.org/mediawiki/2013/9/90/Enlarge.jpg" width="20" height="20" align="right" alt="" /></a></p></div></div></br>
  
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<p>In the meanwhile, mixed microbial communities have garnered much attention owing to their stability, robustness and versatility due to nutrient adaptability, stress resistance and the ability to perform even more complicated tasks than monocultures do. Considering that single-strain MFC faces many practical barriers such as the narrow range of substrates, demanding requirements for environment and relatively slow growth cycle, we are inspired to construct a co-culture MFC system with elaborate labor division. </p></br>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<p>There still remain some obstacles for constructing the co-culture system. The limited information available on the molecules and mechanisms between different microorganisms makes it challenging to establish a harmonious and advantageous relationship. </p></br>
  
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<p>In our system, <span style="font-style: italic">Shewanella</span> function as the exoelectrogens while <span style="font-style: italic">E.coli</span> and <span style="font-style: italic">B.subtilis</span> serve as the fermentation bacteria. In order to better regulate the synthetic microbial consortia, the relationship of material, information and energy is our entry point. For the material relationship, we construct an engineered <span style="font-style: italic">E.coli</span> strain to provide proper amount of carbon source, lactate, for <span style="font-style: italic">Shewanella</span> which have a poor ability of utilizing glucose. In this way, we broaden the spectrum of carbon sources of <span style="font-style: italic">Shewanella</span>. We also regulate the relationship of energy and information, the most effective and visible way for electricity output, by constructing the strain producing riboflavins. Additionally, we attempt to establish a system of lactate sensing and orthogonal targeted protease degradation, which can further become an effective tool in many fields. By reconstruction of the co-culture MFC system, a more efficient and robust system is built up. </p></br>
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<h4>References</h4>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
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<h4>Inspiration</h4>
 
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<p>See how other teams have described and presented their projects: </p>
 
  
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<div id="foot"><p>E-mail: ggjyliuyue@gmail.com |Address: Building No.20, No.92 Weijin road, Tianjin University, China | Zip-cod: 300072</br>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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      </p>
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      <p>Copyright 2015@TJU iGEM Team</p></div>
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Revision as of 14:56, 17 September 2015




Overview

Microbial fuel cells(MFCs) are capable of converting the chemical energy stored in the chemical compounds to electrical energy with the aid of microorganisms. Compared with traditional fossil fuels, MFCs have sorts of advantages, such as no emissions of polluting gas, mild reactive conditions and flexible applications in extreme conditions.



Figure 1. The overall relationship of three kinds of bacteria in our co-culture system.


In the meanwhile, mixed microbial communities have garnered much attention owing to their stability, robustness and versatility due to nutrient adaptability, stress resistance and the ability to perform even more complicated tasks than monocultures do. Considering that single-strain MFC faces many practical barriers such as the narrow range of substrates, demanding requirements for environment and relatively slow growth cycle, we are inspired to construct a co-culture MFC system with elaborate labor division.


There still remain some obstacles for constructing the co-culture system. The limited information available on the molecules and mechanisms between different microorganisms makes it challenging to establish a harmonious and advantageous relationship.


In our system, Shewanella function as the exoelectrogens while E.coli and B.subtilis serve as the fermentation bacteria. In order to better regulate the synthetic microbial consortia, the relationship of material, information and energy is our entry point. For the material relationship, we construct an engineered E.coli strain to provide proper amount of carbon source, lactate, for Shewanella which have a poor ability of utilizing glucose. In this way, we broaden the spectrum of carbon sources of Shewanella. We also regulate the relationship of energy and information, the most effective and visible way for electricity output, by constructing the strain producing riboflavins. Additionally, we attempt to establish a system of lactate sensing and orthogonal targeted protease degradation, which can further become an effective tool in many fields. By reconstruction of the co-culture MFC system, a more efficient and robust system is built up.


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