Difference between revisions of "Team:Freiburg/Protocols"
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| − | < | + | <script type="text/javascript"> |
| − | + | //===================BEGIN:Amazing Bubble Sidebar========================== | |
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| − | + | $(document).ready(function(){ | |
| − | + | // CHANGE THE FOLLOWING ATTRIBUTES // | |
| + | var href_text1='https://2015.igem.org/Team:Freiburg/Notebook', | ||
| + | // Text2 needs no href as it is the actual page // | ||
| + | img_url='https://static.igem.org/mediawiki/2015/9/99/Freibur_icon_notebook_white_03.png', | ||
| + | href_text3='https://2015.igem.org/Team:Freiburg/Materials', | ||
| + | // Text1 needs no text as it is a pic // | ||
| + | text2='Protocols'; | ||
| + | // Text3 needs no text as its always 'next' // | ||
| + | // HOLD ON CHANGING THINGS --JABBERWOCK // | ||
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<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Competent_Cells">Competent Cells</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Competent_Cells">Competent Cells</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cryo_Stocks">Cryo Stocks</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cryo_Stocks">Cryo Stocks</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/IDT_Procedure">gBlock Procedure</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Agarose-Gel">Agarose Gels</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Antibiotics">Antibiotics</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Colony_PCR">Colony PCR</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Competent_Cells">Competent Cells</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Fusion_PCR">Fusion PCR</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Gel_Extraction">Gel Extraction</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Gibson_Assembly">Gibson Assembly</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Isoprop_Precipitation">Isopropanol Precipitation</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Ligation">Ligation</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Miniprep">Miniprep</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/PCR">PCR</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Restriction">Restriction Digest</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Transformation">Transformation</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| − | <div class="content_box2_child" | + | <div class="content_box2_child" id="cell-free_protocol"> |
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<h1>Cell-free Expression</h1> | <h1>Cell-free Expression</h1> | ||
<ul class="horizontal_list"> | <ul class="horizontal_list"> | ||
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/prepLysate">Preparation of an <i>E.Coli</i> lysate for Cell-free Expression</a></li> | |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex">Cell-free Expression general</a></li> |
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex_PDMS">Cell-free Expression on PDMS Flow Cell</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex_PDMS">Cell-free Expression on PDMS Flow Cell</a></li> | ||
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/TerrificBroth">Preparation of Terrific Broth</a></li> | |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/LUC">Luciferase Assay</a></li> |
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/MesurementGFP">Measurement of GFP Expression</a></li> | |
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</ul> | </ul> | ||
</div> | </div> | ||
| − | <div class="content_box2_child"> | + | <div class="content_box2_child" id="surchem_protocol"> |
<h1>Surface Chemistry</h1> | <h1>Surface Chemistry</h1> | ||
<ul class="horizontal_list"> | <ul class="horizontal_list"> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Plasmaactivation">Plasma Activation</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/GOPTS_Surface">GOPTS Surface</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/GOPTS_Surface">GOPTS Surface</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/APTES_PDITC">APTES-PDITC Surface</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Ni_NTA_2">Ni-NTA Surface</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Halo_ABCR">HALO Surface (Silane)</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Halo_ABCR">HALO Surface (Silane)</a></li> | ||
| − | <li><a href=https://2015.igem.org/Team:Freiburg/Protocols/Halo_Piehler>HALO Surface (Jakob Piehler)</a></li> | + | <!--<li><a href=https://2015.igem.org/Team:Freiburg/Protocols/Halo_Piehler>HALO Surface (Jakob Piehler)</a></li>--> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <!--<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Halo_Wiesmueller">HALO Surface (Wiesmüller)</a></li>--> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Glassslide">Glass Slide Preparation (full)</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/SlidePrep">iRIF Slide Preparation (full)</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Spotting">Spotting</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <!--<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Strep_Cy5">Regenration of Tris-NTA Slides</a></li>--> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Moulding_Flowcells">Moulding Flowcells</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/PDMS_PDITC">PDITC Surface on PDMS</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| − | <div class="content_box2_child"> | + | <div class="content_box2_child" id="protpur_protocol"> |
| + | <h1>Protein Purification & Analysis</h1> | ||
| + | <ul class="horizontal_list"> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/AmidoBlack">AmidoBlack</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Bead_Regeneration">Ni-NTA Bead Regeneration</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Buffers">Buffers for Analysis</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cell_Lysis">Cell Lysis</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Desalting">Desalting of Protein Solutions (Columns)</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Dot_Blot">DotBlot</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Freeze_Thaw">Freezing and Thawing Cells</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Microwave_Destaining">Microwave Destaining</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Overexpression">Overexpression</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Periplasmatic_Preparation">Periplasmic Preparation</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Purification">Purification</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/SDS-PAGE">SDS-Page</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Serum_Preparation">Blood Serum Preparation</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Transfection">Transfection</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Western_Blot">Western Blot</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | <div class="content_box2_child" id="irif_protocol"> | ||
<h1>iRIf</h1> | <h1>iRIf</h1> | ||
<ul class="horizontal_list"> | <ul class="horizontal_list"> | ||
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/iRIf_Measurement">iRIf Measurement</a></li> | |
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Protein_Stamping">Protein Stamping</a></li> | |
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Slide_Wash">iRIf Slide Cleaning</a></li> | |
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| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | |
</ul> | </ul> | ||
</div> | </div> | ||
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| + | <div class="content_box2_child" id="interlab_protocol"> | ||
| + | <h1>Interlab Study</h1> | ||
| + | <ul class="horizontal_list"> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/InterLab_Study/Protocol">InterLab Protocol & Worksheet</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
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</div> | </div> | ||
</html> | </html> | ||
{{Freiburg/wiki_content_end}} | {{Freiburg/wiki_content_end}} | ||
Latest revision as of 02:44, 19 September 2015
Cloning
Cell-free Expression
Surface Chemistry
Protein Purification & Analysis
Interlab Study
