Difference between revisions of "Team:Freiburg/Protocols"

 
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<div class="kommentar">
 
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Holla die Waldfee, da fehlen aber noch einige Protokolle: Klonierungen quasi alle (PCR, LIgation, Restriktionsverdau etc pp), cellfree expression quasi alles (Lysat präparation, Expression, Luc und GFP assays - Kokos Bachelor Abreit als Vorbild nehmen?) <br> Materials fehlt auch noch? (oder der link ist noch nicht drin)(ps1209)
 
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Slide clleing und protein stamping zu surface chemistry. Generell sind die Protokolle so gut wie alle nicht wiki geeignet weil man sie nur versteht wenn man weiß was man da macht. Das sind eherArbeitsanweisungen, die man mit ins Lab nimmt aber keine Protokolle die man jemanden gibt damit er es nachkochen kann. Allerdings glaube ich fast nicht, dass wir es noch schaffen die alle bis zum wiki freeze um zu schreiben... (Stefan)
 
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<br> Wie wäre es zumindest die KLonier protokolle vom 2013er wiki zu kopieren? (ps)
 
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     <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cryo_Stocks">Cryo Stocks</a></li>
 
     <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cryo_Stocks">Cryo Stocks</a></li>
 
     <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/IDT_Procedure">gBlock Procedure</a></li>
 
     <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/IDT_Procedure">gBlock Procedure</a></li>
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     <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/PCR_Digest">PCR Template Digest</a></li>
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     <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Agarose-Gel">Agarose Gels</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Antibiotics">Antibiotics</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Colony_PCR">Colony PCR</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Competent_Cells">Competent Cells</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Fusion_PCR">Fusion PCR</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Gel_Extraction">Gel Extraction</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Gibson_Assembly">Gibson Assembly</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Isoprop_Precipitation">Isopropanol Precipitation</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Ligation">Ligation</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Miniprep">Miniprep</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/PCR">PCR</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Restriction">Restriction Digest</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Transformation">Transformation</a></li>
 
   </ul>
 
   </ul>
 
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<div class="content_box2_child" id="cell-free_protocol">
 
<h1>Cell-free Expression</h1>
 
<h1>Cell-free Expression</h1>
 
   <ul class="horizontal_list">
 
   <ul class="horizontal_list">
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex_PDMS">Cell-free Expression on PDMS Flow Cell</a></li>
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<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/prepLysate">Preparation of an <i>E.Coli</i> lysate for Cell-free Expression</a></li>
 
     <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex">Cell-free Expression general</a></li>
 
     <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex">Cell-free Expression general</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex_PDMS">Cell-free Expression on PDMS Flow Cell</a></li>
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<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/TerrificBroth">Preparation of Terrific Broth</a></li>
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    <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/LUC">Luciferase Assay</a></li>
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<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/MesurementGFP">Measurement of GFP Expression</a></li>
 
   </ul>
 
   </ul>
 
</div>
 
</div>

Latest revision as of 02:44, 19 September 2015

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Cloning

Cell-free Expression

Surface Chemistry

Protein Purification & Analysis

iRIf

Interlab Study