Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics"
Vinsonclam (Talk | contribs) |
Vinsonclam (Talk | contribs) |
||
Line 9: | Line 9: | ||
<details> | <details> | ||
<summary>7/26/2015 - 8/1/2015</summary> | <summary>7/26/2015 - 8/1/2015</summary> | ||
+ | <a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/29_July_2015"><li>7/29/2015 - Digestion, Transformation of M2-12(T7), M2-15(1C3)</li></a> | ||
<a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/28_July_2015"><li>7/28/2015 - Maxiprep and BsaI Digestion for M1-AB, BC, CA. Sequencing for M2-12(1C3)</li></a> | <a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/28_July_2015"><li>7/28/2015 - Maxiprep and BsaI Digestion for M1-AB, BC, CA. Sequencing for M2-12(1C3)</li></a> | ||
<a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/27_July_2015"><li>7/27/2015 - M2-12(1C3) Colony PCR, M-15 Amplification</li></a> | <a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/27_July_2015"><li>7/27/2015 - M2-12(1C3) Colony PCR, M-15 Amplification</li></a> |
Revision as of 16:28, 3 August 2015
Spider Silk Genetics Notebook
7/26/2015 - 8/1/2015
7/19/2015 - 7/25/2015
7/12/2015 - 7/18/2015
7/5/2015 - 7/11/2015
6/28/2015 - 7/4/2015
6/21/2015 - 6/27/2015
6/14/2015 - 6/20/2015
5/31/2015 - 6/6/2015
5/24/2015 - 5/30/2015
5/17/2015 - 5/23/2015
5/10/2015 - 5/16/2015
5/3/2015 - 5/9/2015
4/26/2015 - 5/2/2015
4/19/2015 - 4/25/2015
4/12/2015 - 4/18/2015
Contents
Goals
Our goal is to be able to implement Iterative Capped Assmebly (ICA) for use in designing custom spider silk genes. The spider silk protein has a highly repetitive primary structure, and its properties can be changed by changing the sequence and identities of these repeats. We want to be able to fully control the primary sequence of our silk gene, and develop a quick, efficient method of customizing assembly of the spider-silk genes.
Achievements
- We started testing ICA on 6/19/2015
- We successfully constructed biobricks that contain our silk genes and the sticky overhangs. 4/24/2015
- We proved that our designed sticky ends work as expected, and exhibit specific binding, showing that ICA can work in spider silk genes. 5/8/2015 and 5/12/2015
- We have established a protocol generating fragments to be used in ICA.
- We successfully characterized the use of ICA oligos with MaSp AB, BC, CA and showed that they could work in ICA! 6/3/2015
- We successfully made a 3-mer using the ICA protocol on M-270 Streptavidin Beads! 7/7/2015
- Find a list of our designed protocols here!
List of Parts
- [http://parts.igem.org/Part:BBa_K1763002 MaSp2 AB]
- [http://parts.igem.org/Part:BBa_K1763003 MaSp2 BC]
- [http://parts.igem.org/Part:BBa_K1763004 MaSp2 CA]
- [http://parts.igem.org/Part:BBa_K1763009 MaSp2 Seq-AB]
- [http://parts.igem.org/Part:BBa_K1763010 MaSp1 AB]
- [http://parts.igem.org/Part:BBa_K1763011 MaSp1 BC]
- [http://parts.igem.org/Part:BBa_K1763012 MaSp1 CA]
What to accomplish next
We are working on optimizing PCR amplification of MaSp and BsaI digestion so as to get the most amount of working monomers for ICA.--DONE! see hereWe need to characterize our MaSp monomers and how they behave when used with the initiators, terminators, and capping oligos that are also used in ICA.--DONE! 6/3/2015We are going to start actual ICA soon.--STARTED! see 6/19/2015- We are optimizing ICA to get it to work just right!
- Our final goal is to make a several different 15-mers that have different rations of MaSp1 and MaSp2 that we can express and characterize.
Raw lab notebook entries
April | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | 4 | 5 | ||
6 | 7 | 8 | 9 | 10 | 11 | 12 |
13 | 14 | 15 | 16 | 17 | 18 | 19 |
20 | 21 | 22 | 23 | 24 | 25 | 26 |
27 | 28 | 29 | 30 |
May | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | ||||
4 | 5 | 6 | 7 | 8 | 9 | 10 |
11 | 12 | 13 | 14 | 15 | 16 | 17 |
18 | 19 | 20 | 21 | 22 | 23 | 24 |
25 | 26 | 27 | 28 | 29 | 30 | 31 |
June | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | 4 | 5 | 6 | 7 |
8 | 9 | 10 | 11 | 12 | 13 | 14 |
15 | 16 | 17 | 18 | 19 | 20 | 21 |
22 | 23 | 24 | 25 | 26 | 27 | 28 |
29 | 30 |
July | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | 4 | 5 | ||
6 | 7 | 8 | 9 | 10 | 11 | 12 |
13 | 14 | 15 | 16 | 17 | 18 | 19 |
20 | 21 | 22 | 23 | 24 | 25 | 26 |
27 | 28 | 29 | 30 | 31 |
August | ||||||
M | T | W | T | F | S | S |
1 | 2 | |||||
3 | 4 | 5 | 6 | 7 | 8 | 9 |
10 | 11 | 12 | 13 | 14 | 15 | 16 |
17 | 18 | 19 | 20 | 21 | 22 | 23 |
24 | 25 | 26 | 27 | 28 | 29 | 30 |
31 |
September | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | 4 | 5 | 6 | |
7 | 8 | 9 | 10 | 11 | 12 | 13 |
14 | 15 | 16 | 17 | 18 | 19 | 20 |
21 | 22 | 23 | 24 | 25 | 26 | 27 |
28 | 29 | 30 |